Coding

Part:BBa_K1114401:Design

Designed by: Devina Desai   Group: iGEM13_BostonU   (2013-09-07)


This is a MoClo level 0 destination vector.


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 245
    Illegal XbaI site found at 272
    Illegal SpeI site found at 1
    Illegal SpeI site found at 529
    Illegal PstI site found at 284
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 245
    Illegal SpeI site found at 1
    Illegal SpeI site found at 529
    Illegal PstI site found at 284
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 245
    Illegal BamHI site found at 266
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 245
    Illegal XbaI site found at 272
    Illegal SpeI site found at 1
    Illegal SpeI site found at 529
    Illegal PstI site found at 284
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 245
    Illegal XbaI site found at 272
    Illegal SpeI site found at 1
    Illegal SpeI site found at 529
    Illegal PstI site found at 284
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 8
    Illegal BsaI.rc site found at 522


Design Notes

This is a MoClo destination vector containing the lacZ alpha fragment for blue-white screening with fusion sites B on the 5' side and site C on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA.

Summary of modifications from original part:

Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites.


Source

igem kit

References